clean machine
ZBIGNIEW DARZYNKIEWICZ (darzynk@nymc.edu)
Fri, 14 Feb 1997 08:59:31 -0500
Consecutive washes with bleach/detegent - 50% ethanol - 0.5 M NaCl
should remove most of the stuff which attaches to the tubing. Ethanol
dissolves many poorly water soluble (hydrophobic) dyes which may
attach (via precipitation) to the tubing. Since nearly all dyes are
charged molecules, they also attach electrostatically to the tubing.
The best way to remove so attached molecules is to rise ionic
strength (e.g. 0.5 M NaCl), which breaks ionic bonds.
Zbigniew Darzynkiewicz
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
