Re: Transit time

LEKAHN@monsanto.com
Fri, 7 Mar 1997 07:59:38 -0600

The device I know about is made by Cytek (510)657-0102. It is called
a Time Zero Module and I think it's around $5000. I have also seen
some papers in Cytometry over the years with more lab-made devices
that might work for you.

Larry Kahn
Searle Discovery Research

______________________________ Reply Separator _________________________________
Subject: Transit time
Author: owner-cyto-sendout@flowcyt.cyto.purdue.edu at INTERNET
Date: 3/6/97 6:33 PM

Dear Group,

An investigator wants to look (using flow) at Ca++ responses that occur
within seconds (as in 1 or 2 seconds) after the addition of a particular
agent to dissociated endothelial/epithelial cells. Can anyone tell us
the fastest transit time from sample uptake to laser intercept
with either a Coulter Elite or FACSVantage? I also seem to remember a
commercially available device that could be attached to (any?) flow
cytometer and that increased the speed of sample injection. Does anyone
know the particulars about this device - manufacturer, cost, and how well
it works? Can it can be attached to both the Coulter Elite and the
FACS Vantage?

Thanks for any info.

Ray

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Raymond B. Hester, Ph.D.
Flow Cytometry Lab
CSAB 357, Biotechnical Services
The University of South Alabama
Mobile, AL 36688-0002 To err is human -
Email: rhester@jaguar1.usouthal.edu but it feels divine.
Voice:


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu