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> Is the He-Ne path of higher wavelength excitation superior to the
> He-Cd (or Hg arc) path of shorter wavelength excitation?
> Can you give me your tuppence-worth on this?
Generally sppekaing, red excitation has an advantage over UV and
even 488 nm excitation in that there is less autofluorescence from
the biological material that you are trying to study. One of the
exceptions to this rule is plant material of course. Obviously the
best and most flexible path is for new dyes to be developed for all
of the wavelengths that we would wish to use. I'm not convinced that
UV has been overlooked in favour of red excitaion but that may
be because I'm looking around desperately for more red dyes to use
with a laser diode-based instrument at the moment...
> Can you give me your tuppence-worth on this?
Well in local currency that's my 2c (ceiniog, not cents of course!)
Haze
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| Hazel Marie Davey hlr@aber.ac.uk |
|Sefydliad y Gwyddorau Biolegol*Inst. Biological Sciences|
|Prifysgol Cymru * University of Wales|
| ABERYSTWYTH, Ceredigion, CYMRU / WALES SY23 3DA |
| http://pcfcij.dbs.aber.ac.uk/index.htm |
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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