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hope this helps
gary woo
>----------
>From: Meenakshi Roy
>Sent: Tuesday, April 1, 1997 5:30
>To: Cytometry Mailing List
>Subject: monoclonal antibody production
>
>
>Dear flow-ers,
> This is not a directly flow related question. I am planning to produce
>monoclonal antibodies to certain cloned proteins.
> My question is, what form of a protein is most effective for monoclonal
>antibody production? I have been told that fusion proteins dont always
>fold similarly to the native protein. So, monoclonal antibodies that
>recognise the fusion protein, dont always recognise the native protein. On
>the other hand, immunisation with transfectants can result in many
>antibodies that are non-specific to the protein of interest.
> Also, what are the relative merits of HA tagged fusionproetins, or Ig
>chimera fusion proteins, or c-myc tagged fusion protiens for this purpose?
> Any experiences, suggestions shall be highly appreciated,
> Thanks in advance,
>Dr. Meenakshi Roy
>Stanford university
>
>
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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If you have any comments please direct them to
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Phone: (765)-494-0757;
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