I want to determine to ploidy level of some dried herbous plants via
flowcytometry. I checked the material in the light microscope, and the
nuclei seemed to be intact. However, with flowcytometry (razor-blade
technique, DAPI) I found nothing but low intensity, non significant peaks.
My explanation for this behaviour is that not enough fluorochrome got
into the nucleus, but also after longer staining there was no change.
Has anyone got knowledge of a method to stain dried plant materials
(leaves)?
As I am new in the field of flowcytometry, please forgive me my
question if it seems stupid for you; _any_ help (or hint) is
appreciated.
Thanks in advance,
Wolfgang Wlach
Mag. Wolfgang Wlach Institut fuer Pharmakognosie
der Universitaet Wien
Pharmaziezentrum
Tel. +43/1/313 36/8270 Althanstr. 14
Fax. +43/1/313 36/772 1090 Wien