Fluorescein-labeled Peanut Agglutinin(PNA)

YKJEONG@amherst.edu
Tue, 29 Oct 1996 00:16:58 -0500 (EST)

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Hi! Flowers.
I have a plan to do flow cytometry of germinal center B cells stained
with Fluorescein-PNA. But, I do not have information about the suggested
final concentration and amount of the reagent for staining 1 x 10e6 cells.
I got the Fluorescein-PNA (stock conc. 5mg/ml) from "Calbiochem".
If anyone have done this kind of work, please give some tips for my work.
Thank you for your attention.
Sincerely,

Youngkee Jeong
Hybridoma Lab.
Biology Dept.
Amherst College
Ykjeong@amherst.edu
(FAX)(413)542-7955

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