DNA analysis of mollusks -Reply
Charles Kuszynski (CKUSZYNS@MAIL.UNMC.EDU)
Fri, 13 Dec 1996 11:19:46 -0600
I do not know much about the mollusk, but we have routinely examined
blood cells from frogs. These animals can be induced to have 3N
configurations. The protocol we use for staining is the procedure of
Vindelov, LL 1977
Virchows Arch. B Cell. Path.
24:225-242
Basically, it is a 0.1% NP40 with RNAse (3.5 U/ml) and 0.75mg/ml PI in
TRIS.
Charles Kuszynski, Ph. D.
Cell analysis Facility
Univ. Nebr. Med. Cntr.
Omaha, NE 68198-6495
(402) 559-6267