Re: PI staining of PBLs

Dr. Michael G. Ormerod (ormerod@icr.ac.uk)
Thu, 19 Dec 1996 10:27:57 -0500 (EST)

Thank you for the suggestion. We did use fresh blood. The
difference observed was a lymph/gran difference (confirmed by cell
sorting). We will try changing the coagulant.

Michael Ormerod

On Wed, 11 Dec 1996 11:07:41 -0800 "Dr. Philip McCoy" <pmccoy@UMDNJ.EDU> wrote:
> Dr. Michael G. Ormerod ;
>
> What was your anticoagulant and how old was the blood? MOst likely you
> have a lymph/gran difference. Try using real fresh blood (<2hrs old) in
> Na-heparin. Good luck!
>
> Phil
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