Computer Resources.

In addition to the three dedicated computers configured with the instruments, the HSS Flow Core provides three remote Macintosh workstations for analysis of collected data. These are located on the ground floor of the HSS Research Building two of these were recently relocated from 3rd and 4th floor locations). All of these workstations are equipped with magneto-optical cartridge drives and inkjet printers and are networked to the HSS ethernet to facilitate transfer of files from the 2nd floor instrument workstations. All instrument and remote workstations run the most recent update of CellQuest (version 3.1) as well as the DNA cell cycle analysis program ModFit ver. 2.0. The new flow analysis program FlowJo (developed at Stanford University) is being tested on one of the ground floor workstations.

The Flow Core also has a 200 Mhz Pentium PC that can accept flow data files from Mac (via the program MacOpener), for users who would prefer their data in a PC-accessible form. This PC has a 230 Mb magneto-optical cartridge and a 100 mb ZIP drive, both for transfer and conversion of files from the Mac workstations and for copying to the computer medium of the user's choice. This PC also has a writeable CD-ROM drive for data archiving. In addition, we maintain a large collection of PC-based flow cytometry and graphics applications. These include:

• WinList ver. 3.0. A flow analysis program particularly useful for batch analysis of large numbers of files.
• WinMDI ver. 2.5. An freeware flow analysis program written by Dr. Joe Trotter at Scripps. Has nice graphics. You can also get this program for your own PC - just follow this link (or ask us for it).
• PC-LYSYS ver. 1.0. A Becton-Dickinson program for analyzing flow data on the PC.
• HP-Reader. A program for converting flow data files from HP to PC format. Useful for getting at data stored under the old HP UNIX system (which Becton-Dickinson used to use).
• Cyclred ver. 1.1.1. A DOS-based cell cycle analysis program designed by Drs. James Watson and Michael Ormerod in Great Britain. You can get this program for your own PC, too - follow this link.
• HP LIF Utilities. A program for converting and reading old HP UNIX flow data files - and, you guessed it, it's available from The University of Massachusetts free flow software site, maintained by Dr. Eric Martz. Other old flow programs are there for free, too.

We also have image editing and drawing programs to convert raw flow data into clear, polished figures for publication. These include:

• Corel Photo-Paint (image editing)
• Microsoft PowerPoint (construction of figures and slides)
• Corel Draw (construction of figures and slides)

We also have the following on-disk and CD-ROM resources:

• Linscott’s Directory on Disk 1996-97
• Immunology Methods Manual on CD-ROM (Lefkovitz)
• Immunobiology Bookshelf on CD-ROM (Janeway)
• Sigma Catalog on CD-ROM
• The Purdue Cytometry CD-ROMs volumes 1, 2 and 3 (includes the entire Purdue Cytometry e-mail archives from 1992 to present on CD-ROM)
• A WWW connection for access to other flow-related sites

Back to the HSS Flow Cytometry Core Facility Home Page.