Welcome to the HSS Flow Cytometry Core
Facility!
The HSS Flow Cytometry Core Facility provides flow cytometric
analysis and fluorescence activated cell sorting services to the
HSS research community and to investigators from other
metropolitan area research universities and institutions. Our
primary purpose is to promote the use of flow cytometry as a
research tool for the investigation of arthritis and other
muscloskeletal disorders that fall under the mission and
expertise of the Hospital for Special Surgery. Both HSS and
outside investigators are welcome to use our facilties at a
reasonable cost.
In addition to providing flow cytometry services, we also
maintain an internal research program directed at developing flow
cytometric technologies in support of the investigators who
utilize our facility. Our goal is to make this facility an active
collaborator in your biomedical research projects. We represent a
strong resource both in terms of instrumentation and expertise.
Please feel free to contact us with
your research questions and needs.
Information about the Flow Core...
Temporarily short of research funding? We have a program for
"loaning" instrument time to new investigators
preparing their first grant, or to established investigators who
have lost their funding and are in the process of reacquiring it.
Click here for more information.
Our B-D
FACSVantage cell sorter flow nozzle and sample stream illuminated
by an argon laser. For more information on our B-D Vantage, click here.
Projects and collaborations going on in the
Flow Core...
- Cytoenzymology
and flow cytometry.
Development of flow assays for acid and alkaline
phosphatases, NADPH dehydrogenases and other enzymes
relevant to cellular differentiation.
Recent facility developments...
-
Due to increased operating costs, we are increasing our
hourly instrument rates for both HSS and outside users as
of January 1st, 1998. Click here for an updated
fee schedule.
- We're now including flow protocols and
sample data on our page, many developed in collaboration
with our users. If you want to contribute one of your
protocols or some typical experimental data illustrating
what you're using the facility for, let us know.
- The B-D FACSCalibur is fully operational
and is being routinely used for four-color analysis. Let
us help you with protocol design. Click here for an
example of four-color analysis on the FACSCalibur. We
also have limited quantities of PerCP, APC, Cy5 and
AMCA-conjugated reagents available for pilot four- and
five-color experiments - click here to find out
more.
Our CD-ROM archiving system is
operational. Every month your data will be permanently
archived onto writeable CD-ROM. This represents a
considerable improvement over our old tape system, both
in ease of access and durability. You can also prepare
your own archive CD of previous data. Talk to us for
details.
Links...
...to flow cytometry sites in the USA and
Canada...
- Purdue Cytometry Laboratory website at Purdue
University (one of the first
and best flow cytometry facility homepages, home of Dr.
J. Paul Robinson and company)
- Purdue Cytometry e-mail list (THE best source for flow information, a model
for all e-mail discussion groups)
- TSRI Cytometry Software Page (the Scripps Institute flow page, home of Dr.
Joe Trotter and his freeware flow analysis program
WinMDI)
- The University of Massachusetts-Amherst Flow
Cytometry Facility (home of Dr.
Eric Martz)
- Catalog of Free Flow Cytometry Software (Dr.
Eric Martz, University of Massachusetts) (a lot of free flow software here)
- Salk
Flow Cytometry Laboratory
- Center for Quantitative Cytometry Information
Network
- Cooper Cytometry Home Page (University of
Medicine and Dentistry of New Jersey)
- The Johns Hopkins University: Center for
Analytical Cytology
- Cornell University Flow Cytometry and Imaging
Facility Home Page (Ithaca)
- University
of California-San Francisco Division of Molecular
Cytometry
- The Flow Cytometry Consortium Webpage (NIH)
- The University of Washington Cell Analysis
Facility
- The University of Georgia Cell Analysis Facility
- Los Alamos National Laboratories Life Sciences
Division - L-5 Flow Cytometry Laboratory (a national repository for flow cytometry
information)
- Los
Alamos National Laboratories National Flow Cytometry
Resource Home Page (protocols,
sample data, etc.)
- The
University of Texas Medical Branch Molecular Cytometry
Facility
- Texas A & M University Department of
Veterinary Pathobiology Flow Cytometry Core Laboratory
- The University of Florida Cytometry Core (a good educational page - they are starting a
listing of antibody species cross-reactivities)
- Dana-Farber
Cancer Institute Core Cytometry Facility
- The MIT Flow Cytometry Core Facility
- EOHSI/The Cancer Institute of New Jersey Flow
Cytometry/Image Analysis Core Facility (Rutgers)
- The University of Alabama Cell Marker Shared
Facility
- The University of Maryland Center for
Fluorescence Spectroscopy
- The
Athey Laboratory (The
University of Michigan Digital Microscopy Facility, home
of Dr. Brian Athey)
- Iowa State University Cell and Hybridoma
Facility (they have a new
Coulter Elite-ESP (5 lasers, 7 colors), the lucky dogs!)
- The University of Connecticut Health Center's
Flow Cytometry Facility (home
of Gene Pizzo)
- The
University of Connecticut Center for Biomedical Imaging
Technology
- Yale University Center for Cell Imaging
- Yale Cancer Center Flow Cytometry/Cell Sorting
Shared Resource
- Albert Einstein University FACS Facility
- Mount Sinai School of Medicine Flow Cytometry
Core
- Roswell Park Laboratory of Flow Cytometry,
Buffalo, NY (home of Carleton
C. Stewart)
- Michigan State University Flow Cytometry
Facility (home of Louis King)
- Princeton University Flow Cytometry Core (Beavis on-line!)
- Tulane University Flow and Image Cytometry
Facility
- Duke University Comprehensive Cancer Center Flow
Cytometry Facility
- The University of Illinois Flow Cytometry
Facility
- SUNY
Stony Brook University Microscopy Imaging Center
- The University of Pittsburgh Medical Center
Clinical Flow Cytometry Laboratory
- Maine Medical Center Hematology Research
- Wistar Institute Flow Cytometry Facility Home
Page
- The University of British Columbia Department of
Physiology Confocal Microscopy Laboratory
...to flow cytometry sites in Europe and
Asia...
...to cytometry societies (both in North
America and abroad)...
...to cytometry-related companies...
- Accurate (tons of
antibodies and other reagents, including a lot of unusual
stuff, often marketed for other companies)
- Affinity Bioreagents
(steroid and stress protein antibodies and probes)
- Aldrich (chemicals)
- Amersham (secondary antibodies and the Cy series of
fluorochromes, including conjugation kits)
- Atto
Instruments, Inc. (analytical
microscopy)
- Bachem (reagents, including caspase inhibitors)
- Becton-Dickinson
Immunocytometry Systems
(instruments and clinical antibodies, supplier of
PerCP-conjugated reagents)
- Biomeda (fluorochrome-conjugated antibodies,
particularly phycoerythrin)
- Biomol (lots of reagents for signal transduction)
- Bio-Rad (not much for flow, but they make an odd sort
of cytometer for analyzing bacteria (the Bryte))
- BioSource (antibodies for cytokine detection by flow,
they also have Tago Immunochemicals products)
- Calbiochem (signal tranduction reagents - high quality
indo-1 and fluo-3 for calcium detection)
- Caltag (anti-human and anti-mouse antibodies - they
have APC, Cy5 and Cy3 conjugates)
- Coherent (lasers,
lasers, lasers)
- Coulter (flow cytometers and sorters - they have a new
line of fluorescent cytoenzymology reagents for flow -
interesting stuff)
- Cytek (they make flow cytometer add-ons, including
our Time Zero kinetic module, absolute count devices and
an automatic cell washer)
- Cytomation (manufacturers of the MoFlo sorting system -
can sort up to 25,000 events/second! - they also market
Partec flow cytometers)
- Dako (lots of anti-human antibodies)
- Enzyme
Systems Products (fluorogenic
enzyme substrates, caspase inhibitors, etc.)
- Exalpha (anti-human antibodies, including panels)
- FlowJo (a new flow cytometry program developed at
Stanford)
- Fluka (chemicals)
- Gibco-BRL (source of Green Fluorescence Protein for flow,
PE-Texas Red conjugates, etc.)
- Immune
Source (lots of antibodies for
cytokines, AIDS, multicolor panels)
- Immunotech (Coulter owns them, good selection of
anti-human antibodies)
- Jackson
ImmunoResearch
(well-characterized secondary antibodies for multicolor
labeling, many Cy2, Cy3, Cy5 and AMCA conjugates found
almost nowhere else, and good technical info)
- Lab
Vision, Inc. (a source for
unusual and hard-to-find antibodies)
- Laser
Innovations (they sell and
service Coherent lasers)
- Leinco (antibodies for flow cytometry, including Image
Blue conjugates (a fluorochrome like Cascade Blue))
- Leica (the microscope people)
- Medical
Science Associated (software
for flow cytometry)
- Melles
Griot (they make filters for
fluorescent microscopes and flow cytometers)
- Molecular
Probes (everything fluorescent!
- their catalog is an education in itself, and is
available on-line)
- Nikon (some other
microscope people)
- Olympus (more
microscopes)
- Omega (filters for
flow, including useful technical info on laser excitation
wavelengths, filter wavelengths, GFP, etc.)
- OncoImmunin (novel
fluorescent caspase substrates for flow cytometry - we
are testing these)
- Opti-Scan (confocal microscopy systems)
- Partec (a German flow cytometer manufacturer)
- Pharmingen (one of the
last great manufacturers of anti-mouse antibodies, they
have CyChrome (PE-Cy5) and APC conjugates and lots of
apoptosis-related antibodies, and good TUNEL and annexin
V apoptosis assays - are now an affiliate of
Becton-Dickinson and now PerCP-conjugated antibodies as a
result)
- Phoenix
Flow Systems (they recondition
old Ortho flow cytometers and sell flow software,
apoptosis kits, etc.)
- Polysciences
(fluorescent beads and solid protein substrates for
bead-based flow assays)
- ProZyme (PE and APC
conjugation kits for antibodies and other proteins)
- Research Diagnostics, Inc. (antibodies and research drugs)
- Research Organics
(XRITC, Cy3 and Cy5 linker kits)
- Riese
Enterprises (they sell
BioSource sheath buffer, red blood cell standards, etc.)
- Santa Cruz
Biochemicals (cell cycle
antibodies)
- Scanalytics
(fluorescence imaging microscopes)
- Sigma (biochemicals,
good selection of secondary antibodies, they sell PKH2 and PKH26, those
lipophilic membrane markers developed by Zynaxis Cell
Sciences)
- SoftFlow Hungary (a
flow cytometry software development company in Eastern
Europe, they wrote the FCAP flow analysis software for
the Mac, a pretty good program)
- Spherotech (calibration particles, including Rainbow
beads)
- Synthetic Genetics
(fluorescent oligos)
- Verity
Software (several good software
packages, including WinList and ModFit, both of which we
have)
- Zeiss (and even more microscopes, including confocal)
- Zymed (apoptosis and signal transduction antibodies,
more immunocytochemistry-oriented)
...and to other useful sites.
- The OSHA Guidelines for Handling of Bloodborne
Pathogens (required reading if
you analyze biohazardous samples)
- Fluorochrome emission/excitation spectra (from Israel, I think, useful information on
excitation/emission characteristics of some less common
fluorochromes)
- Journal of Fluorescence
(often the first place new fluorochromes appear)
- Bioconjugate Chemistry (an
ACS journal)
- ESACP Analytical Cellular Pathology (a European pathology journal with a lot of
flow)
- American
Type Culture Collection (ATCC)
- Materials and Cultures Collections Menu (sponsored by the MRC, UK, a collection of cell
and tissue repositories)
- Frontiers
in Bioscience List of Knockout Mice
- Table
of the CD Antigens (courtesy of the Department of
Crystallography, Birbeck College, London, UK)
- The Antibody
Resource Page (THE source for antibody information,
sources, pictures, etc - they have a comprehensive list
of on-line reagent search indexes)
- Moscow State University
- Russian Academy of Sciences
- Institute
of Protein Research, Puschino, Russian Federation