Re: Eosinophils
Howard Shapiro (hms@shapirolab.com)
Wed, 24 Jan 1996 20:32:17 -0500
The easiest way to positively discriminate and sort eosinophils, first
described by Bart DeGrooth, Leon Terstappen et al in Cytometry in the
mid-1980's, involves no reagent at all. Substitute a polarizing filter
(plastic is fine) for the filter in one fluorescence channel; pur another
polarizing filter in front of your orthogonal scatter detector. Running
beads, rotate one filter for maximum ("polarized", i.e., same plane as the
excitation beam) and the other for minimum ("depolarized", i.e.,
perpendicular to the plane of the excitation beam) signal. Eosinophils of
virtually all mammalian species examined to date have a higher ratio of
depolarized to polarized scatter and show up as a discrete cluster; they can
be sorted easily.
--Howard Shapiro
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
