Purdue University Cytometry Laboratories Overview
Department of Basic Medical Sciences, School of Veterinary Medicine,
Purdue University
The Purdue University Cytometry Laboratories Facility
People who have Homepages in this lab:
This Facility Homepage| J.Paul Robinson| Jim Whitfield
| Karen Cornell| Sherry Harbin
Description of Facility
The Purdue University Cytometry Laboratories (PUCL)
is an advanced Cytometry instrumentation
facility. The facility works with over 40 professors
from 15 different departments at Purdue
University and many industrial scientists from pharmaceutical
companies, medical product companies, and
high technology engineering companies.
(List of projects)
The Purdue University Cytometry Laboratories was established
to provide both the university and industry with a state-of-the-art
facility for analytical cytology, instrumentation engineering, and
computer-assisted diagnostics through expert system development.
The strong links that exist between the Cytometry Laboratories and
the Schools of Engineering prompted the establishment of the
Engineering Development Laboratory.
This laboratory's purpose is to develop new instrumentation and
to apply leading edge engineering technology to cytometry. Expertise
in signal processing, optics, computer engineering, data analysis,
networking, and expert systems is available through
affiliated faculty and graduate students.
A new Optical Engineering Instrumentation and Cell Biology
program has recently been established. This program funds
graduate/postgraduate students in the areas designated
within the Engineering Development Laboratory. Scholarships
are available through this program for exceptional students.
The application of expert systems and neural networks to
disease diagnosis and chemotherapeutic monitoring is an
important development area. We are interested in studying
analytical techniques for cytometric data handling and
analysis leading to exciting capabilities using expert
systems.
The facility has a strong commitment to biological
research, particularly in the area of immunology. Research
programs in immune deficiency, trauma & shock, as well
cancer research are supported in the facility.
The facility is rapidly developing an interest in
Microbial Cytometry. The application of flow cytometry
to the field of microbiology is clearly becoming more important. We are
currently working toward a new program in this area.
The facility has an interest in developing computer interactive teaching and instructional
materials. We have begun this process by developing a specialty CD-ROM which
links the field of cytoemtry electronically. Information about this project is available
(Educational Activities).
One related activity has been the compilation of
methodologies for flow cytometry. In this regard, the
Current Protocols in Cytometry.
In addition, this laboratory has been in volved in other significant publications
such as Methods in Cell Biology Vol 41 and
Methods in Cell Biology, Volume 42.
The facility is equipped with nearly $2 million worth of
state-of-the-art instrumentation including 4 high-speed
cell sorters as well as a benchtop analyzer (Bio-Rad BRYTE HS flow cytometer)
[FLOW CYTOMETRY].
For Image analysis, a Bio-Rad MRC 1024 UV/VIS confocal microscope, and a DVC
direct view confocal scope are available both mounted on inverted Nikon 300
scopes
[CONFOCAL MICROSCOPY & IMAGE ANALYSIS].
The computing area contains 20 networked computers as well as laser
printers, CODONICS NP 1600 dye-sub color printer, optical storage,
and digital camera capabilities for color graphics.
Two Silicon Graphics Indigo 2 computers run VoxelView for
3D image reconstruction.
The facility is linked to Bitnet and Internet via fiber optic cable, for
connection worldwide. The Flow Cytometry Mailing list cytometry@flowcyt.cyto.purdue.edu
is operated from this facility. This links approximately 1250 cytometrists from around the world.
If you would like to join the user group or request further information you can do so now
[email to Cytometry
User Group Administrator].
The mailing list is a discussion group in the area of flow cytometry and has
been operating since 1989. An FTP site is available to send and receive cytometry
related information [FTP
to PURDUE now].
For information contact J.Paul Robinson, Ph.D, Director PUCL, 1515 Hansen B050,
Purdue University, West Lafayette, IN 47907-1515, U.S.A. Phone: (317) 494-0757;
Fax: (317) 494-0517: Send an email request now to J. Paul Robinson:
robinson@flowcyt.cyto.purdue.edu
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
