FIX & PERM®

Applications Guide to Intracellular Flow Cytometry

FIX & PERM® reagents are intended for fixing cells in suspension with Reagent A and then permeabilizing the cells with Reagent B. This procedure gives antibodies access to intercellular structures and leaves the morphological scatter characteristics of the cell intact. The unique formulation of FIX & PERM® reagents reduces background staining and allows simultaneous addition of permeabilization medium and fluorochrome labeled antibodies.

USE OF FIX & PERM® REAGENTS

STAINING PROTOCOLS

APPLICATIONS USING FIX & PERM®

TYPICAL FLOW CYTOMETRIC PATTERNS

SELECTED REFERENCES

Reagents Available From CALTAG

FIX & PERM® Permeabilization Kits

COMBI - IC® Monoclonal Antibodies:

For Intracellular Antigen Detection

For Intracellular Murine Cytokine Detection

For Intracellular Human Cytokine Detection

The FIX & PERM® reagent system and the COMBI-IC® antibodies were developed by AN-DER-GRUB Bioresearch, Austria. These products are offered worldwide as a partnership between CALTAG and AN-DER-GRUB.

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu