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Purdue University Cytometry Laboratories Overview

Department of Basic Medical Sciences, School of Veterinary Medicine, Purdue University


NEW INFORMATION ...... Cytometry Meetings in the next months...
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The Purdue University Cytometry Laboratories Facility


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Description of Facility
The Purdue University Cytometry Laboratories (PUCL) is an advanced Cytometry instrumentation facility. The facility works with over 40 professors from 15 different departments at Purdue University and many industrial scientists from pharmaceutical companies, medical product companies, and high technology engineering companies. (List of projects)

The Purdue University Cytometry Laboratories was established to provide both the university and industry with a state-of-the-art facility for analytical cytology, instrumentation engineering, and computer-assisted diagnostics through expert system development.

The strong links that exist between the Cytometry Laboratories and the Schools of Engineering prompted the establishment of the Engineering Development Laboratory. This laboratory's purpose is to develop new instrumentation and to apply leading edge engineering technology to cytometry. Expertise in signal processing, optics, computer engineering, data analysis, networking, and expert systems is available through affiliated faculty and graduate students.

A new Optical Engineering Instrumentation and Cell Biology program has recently been established. This program funds graduate/postgraduate students in the areas designated within the Engineering Development Laboratory. Scholarships are available through this program for exceptional students.

The application of expert systems and neural networks to disease diagnosis and chemotherapeutic monitoring is an important development area. We are interested in studying analytical techniques for cytometric data handling and analysis leading to exciting capabilities using expert systems.

The facility has a strong commitment to biological research, particularly in the area of immunology. Research programs in immune deficiency, trauma & shock, as well cancer research are supported in the facility.


Methodology development and testing have become an important part of the facility. The facility is committed to developing educational material for learning and teaching of flow cytometry and confocal microscopy technologies (Educational Activities). One related activity has been the compilation of methodologies for flow cytometry. In this regard, the Handbook of Flow Cytometry Methods was spearheaded from this facility. New methodologies in both flow cytometry and confocal microscopy are of interest to the faciltiy for the compilation of Current Protocols in Cytometry. Scientists interested in submitting protocols please follow the instructions provided. Current Protocols in Cytometry

The facility is equipped with nearly $2 million worth of state-of-the-art instrumentation including 4 high-speed cell sorters as well as a benchtop analyzer (Bio-Rad BRYTE HS flow cytometer) [FLOW CYTOMETRY]. For Image analysis, a Bio-Rad MRC 1024 UV/VIS confocal microscope, and a DVC direct view confocal scope are available both mounted on inverted Nikon 300 scopes [CONFOCAL MICROSCOPY & IMAGE ANALYSIS]. The computing area contains 20 networked computers as well as laser printers, CODONICS NP 1600 dye-sub color printer, optical storage, and digital camera capabilities for color graphics. Two Silicon Graphics Indigo 2 computers run VoxelView for 3D image reconstruction.

The facility is linked to Bitnet and Internet via fiber optic cable, for connection worldwide. The Flow Cytometry Mailing list

(cytometry@flowcyt.cyto.purdue.edu)
is operated from this facility. If you would like to join the user group or request further information you can do so now [email to Cytometry User Group Administrator]. The mailing list is a discussion group in the area of flow cytometry and has been operating since 1989. An FTP site is available to send and receive cytometry related information [FTP to PURDUE now].
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For information contact
J.Paul Robinson, Ph.D, Director PUCL, 1515 Hansen B050, Purdue University, West Lafayette, IN 47907-1515, U.S.A.
Phone: (317) 494-0757; Fax: (317) 494-0517: Send an email request now to J. Paul Robinson: robinson@flowcyt.cyto.purdue.edu