Purdue Cytometry CD ROM

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Sorting Applications

Chromosome Analysis & Sorting

Hoechst 33258 vs Chromomycin A3 flow karyotype for a karotypically normal human male. The numbers identify the chromosome producing peaks.

Using the ZOOM feature of the LYSYS II software it is possible to view an exploded portion of a display. This is taken from the previously displayed dotplot above.

Sorting for Subsequent in situ Hybridization

Cells from male peripheral blood were sorted using scatter gates, then spun onto glass slides and hybridized with a probe to detect the Y chromosome. The probe is biotinylated with the streptavidin FITC second step. Nuclei were counter stained with PI. Fig 2a, 2b and 2c represent lymphocytes, monocytes and granulocytes respectively.

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HOMEPAGE or Immunophenotyping applications, or DNA Applications or Chromosome Sorting or Functional Studies

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This CD was produced by the staff of the Purdue University Cytometry Laboratories to promote the interchange of cytometry information. If you want more information please send a message to J.Paul Robinson at robinson@flowcyt.cyto.purdue.edu or by mail, J.Paul Robinson, Ph.D, Professor & Director PUCL, 1515 Hansen B050, Purdue University, West Lafayette, IN 47907-1515, U.S.A. Phone: (317) 494-0757; Fax: (317) 494-0517