Re: Eosinophils

Dr. Gregor Rothe (Gregor.Rothe@klinik.uni-regensburg.de)
Wed, 24 Jan 1996 19:11:01 +0100

> Date: Wed, 24 Jan 1996 00:07:15 +0100
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> From: Janek Brozek <udbrozek@cyf-kr.edu.pl>
> Subject: Eosinophils

> Dear Flowlanders,
>
> Please excuse me my ignorance, but if you would be able to tell me if there
> is any antigen allowing POSITIVE selection and sorting of eosinophils... To
> my knowlege there's none, but ....
>
> Masses of thanx in advance!
>
> Janek
>
> _______________________________________________________
>
> Janek Brozek e-mail: udbrozek@cyf-kr.edu.pl
>
> 2nd Dept of Internal Medicine
> Allergy and Immunology Clinic
> Jagiellonian University
> Krakow, Poland
>
>

Try dual colour staining with CDw65 and CD16. Eosinophils
are slightly brighter than neutrophils in CDw65 but almost
negative for CD16.

Gregor Rothe
*************************************************************************
Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, F.R. Germany

Tel. +49 (941) 944-6204
Fax +49 (941) 944-6202

Internet: Gregor.Rothe@klinik.uni-regensburg.de
*************************************************************************


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